Fig. 4.

Absence of CysLT₂R normalizes tumor vasculature and inhibits tumor metastasis. LLC cells (2 × 10⁶ in 100 μL of PBS) were injected s.c. in both flanks of the indicated mouse groups. On day 21, mice were euthanized and lungs were harvested for histological studies. (A) H&E staining of the lungs showing metastasis. (Scale bar: 200 μm.) Metastasis foci (B) and quantification of the metastasis area (C) are shown. (D) Representative immunofluorescence images of tumors of the indicated mouse groups stained with an endothelial marker (CD31, green) and pericyte marker (NG2, red), and merged (n = 10–15 per group). (Scale bars: 100 μm.) Quantification of tumor vessel density (E; CD31, green) and pericyte coverage (F; NG2, pericyte in red/CD31, EC in green) from the indicated mouse groups. (G) Tumor sections from the indicated mouse groups injected with Texas (TX) Red-conjugated dextran and stained with CD31, showing the leakiness of the vessels (n = 10–12 per group). (Scale bars: 100 μm.) (H) Quantification of Texas Red-conjugated dextran leakage into the tumor tissue. Data are shown as mean ± SEM. *P < 0.05; ***P < 0.001; NS (nonsignificant) determined by one-way ANOVA with a Tukey post hoc test.