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In situ visualization of crucial metabolite and metabolic enzyme in the proline biosynthesis pathway. (A) MS image of proline. (B) MSI and H&E overlay image. (C) Proline levels in cancer and paired epithelium and muscle tissues from 256 ESCC patients (means ± SD). ***P < 0.001. (D) H&E image of ESCC tissue section. (E) Expression of PYCR2 in different regions of ESCC tissue section. CT, cancer tissue; ET, epithelial tissue; MT, muscular tissue.
