Fig. 1.

NG-TAS workflow and alignment pipeline. a NG-TAS workflow. Primers were designed and multiplexed for direct amplification in cfDNA obtained from plasma using Fluidigm Access Array™. The PCR products were harvested and barcoded in a subsequent PCR reaction; the samples were pooled and size selected for sequencing on an Illumina Hi-Seq 4000. b Schematic representation of the computational pipeline for reads alignment, filtering and annotation