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. 2018 Dec 19;24:9251–9258. doi: 10.12659/MSM.911594

Figure 3.

Figure 3

CuB induces caspase-dependent apoptosis in CRC cells. (A) SW620 cells were incubated with various concentrations of CuB for 24 h. Cells were examined by DAPI staining. Arrows: apoptotic nuclear morphology. (B, C) SW620 and HT29 cells were treated with increasing concentrations of CuB for 24 h, and cell apoptosis was analyzed by annexin V/PI staining and flow cytometry. (D, E) SW620 and HT29 cells were treated with increasing concentrations of CuB for 24 h. Western blot analyses were carried out using the indicated antibodies. * P<0.05; ** P<0.01 vs. 0 μM (for B, C).