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HEY1 was a target of miR-769. (A) Wild-type and mutant luciferase activity reporter plasmids were constructed. (B) Luciferase activity was detected in HCT116 and SW480 cells transduced with miR-769 or miR-NC. (C, D) Relative mRNA and protein levels of HEY1 were measured by qRT-PCR and western blot. * P<0.05 and ** P<0.01.
