Figure 4.

CD47 regulates global gene expression in naive NK cells. Lin⁻NK1.1⁺NKp46⁺ cells were sorted from spleens of WT and Cd47^−/− mice. Total RNA was isolated from sorted cells, fragmented, cDNA library was prepared followed by paired end sequencing. (A) PCA comparing global gene expression between WT and Cd47^−/− naïve NK cells. (B) Volcano plot shows that many genes are significantly upregulated in naïve Cd47^−/− compared to naïve WT NK cells. (C) Boxplots show log2 expression of Klrb1c, Myc, Klf4, and Mki67 in WT and Cd47^−/− naïve NK cells. (D) GSEA plots showing enrichment of early effector, late effector, sustained effector, interferon, memory and proliferation signature genes in naïve Cd47^−/− over WT NK cells. Data were derived from an experiment involving four mice per group.