Figure 5.

CD47 limits LCMV infection in mice. (A) WT and Cd47^−/− age and sex-matched mice were infected with LCMV Cl-13 (i.v. injection of 2 × 10⁶ pfu/mouse), and timeline shows infection and analysis. (B) Viral titers were quantified in serum of LCMV Cl-13 infected mice on the indicated days (D) post infection. On day 25 and day 46 of LCMV Cl-13 infection, mice were euthanized, and single cell suspension of spleens were stained for Aqua live/dead, Lin (CD4, -CD8, -TCRβ, -B220, -Gr1, and -Ter119), NK1.1, NKp46, and PD-1. Cells were then fixed, permeabilized and intracellularly stained for Eomes, T-bet, granzyme B (GzmB) and Ki-67. (C–F) Representative contour plots (values indicate percentage of live cells) and frequency of Lin⁻NK1.1⁺ cells and Lin⁻NK1.1⁺NKp46⁺ cells within the spleens on day 25 post infection are shown; (G–K) Frequencies of Eomes, T-bet, PD-1, GzmB and Ki-67 positive NK cells are plotted. (L–O) Representative contour plots (values indicate percentage of live cells) and frequency of Lin⁻NK1.1⁺ cells and Lin⁻NK1.1⁺NKp46⁺ cells are shown within the spleens on day 46 post infection and (P–T) Frequencies of Eomes, T-bet, PD-1, GzmB and Ki-67 positive NK cells n = 5. Data obtained from representative of two experiments involving 4–5 mice per experiment (C–T), and more than five experiments comprised of four to seven mice (B) per group. (Mean ± SEM).