Figure 6.

CD47-deficiency limits the transcriptional response during LCMV infection in mice. Lin⁻NK1.1⁺NKp46⁺ cells were sorted from spleens of uninfected and LCMV-infected WT and Cd47^−/− mice. Total RNA was isolated, fragmented, cDNA library was prepared followed by paired end sequencing. (A) PCA on global transcriptome data shows four distinct clusters of naïve WT (purple), naïve Cd47^−/− (blue), LCMV Armstrong infected WT (green) and LCMV Armstrong infected Cd47^−/− NK cells (red). (B) Volcano plots show broad significant upregulation and downregulation of NK cell gene expression in infected WT and Cd47^−/− mice compared to their uninfected counterparts. (C) Expression pattern of Cd47, Hif1a, Irf7, Gzmb, Gzmc, and Sirpa are indicated within the volcano plot showing upregulation and downregulation of NK cell gene expression in infected Cd47^−/− vs. infected WT mice. (D) GSEA shows significant negative enrichment of naïve, effector and interferon signature genes in Cd47^−/− infected compared to WT infected NK cells. Data were derived from an experiment involving four mice per experiment.