(A) Immunofluorescence of mCD8-GFP (green) and Brp (magenta) in the indicated driver lines in the brain and thoracic ganglion. (B) Mean spontaneous turning rate of flies of the indicated genotype at 19°C (blue) and 34°C (orange); each dot represents one fly. (Black bars: mean ± SEM, n = 7 - 18. Genotypes labeled with different letters have saccade rates that are significantly different at 34°C, Mann-Whitney U Test with Bonferroni correction. * p < 0.05, *** p < 0.001, Wilcoxon Signed Rank Test). An outlier NP0212 > Shits, elav-GAL80 fly that had a spontaneous saccade rate of 91.6 turns/min and an outlier NP0212 > Shits, tsh-GAL80 fly that had a spontaneous saccade rate of 89.4 turns/min at 34°C are not shown on the plot.