Extended Data Figure 7 |. Co-labelling of P-EN1 and P-EN2 driver lines.

A–c, Maximum z-projection of a brain with 12D09-driven neurons expressing GFP and VT032906-driven neurons expressing tdTomato. a, GFP (12D09) signal. b, tdTomato (VT032906) signal. c, Composite of a and b. Physiological experiments suggest that VT032906 primarily labels P-EN1 neurons, whereas 12D09 primarily labels P-EN2 neurons (Fig. 3, Extended Data Fig. 8b, d). As expected, most P-EN neurons are primarily labelled by one of the two drivers, but some neurons are labelled by both (examples denoted with asterisks). D–f, Same as a–c but with VT020739-driven neurons expressing tdTomato. Physiological experiments suggest that both 12D09 and VT020739 primarily label P-EN2 neurons (Fig. 3, Extended Data Fig. 8c, d). As expected, almost all labelled P-EN neurons are labelled by both P-EN2 drivers. P-ENs often showed fluorescent signals whose strength varied across glomeruli, which could reflect varying innervation densities across the bridge. They could also reflect incomplete targeting of P-ENs by our driver lines.