. Surface CCR2 on CD14+CD16+ monocytes was determined by
staining freshly isolated PBMC for human CD14, CD16, and CCR2, or with isotype matched
control antibodies. CCR2 MFI was calculated by subtracting the MFI of the IgG2b isotype
matched control staining on CD14+CD16+ monocytes from the MFI of
CCR2 on CD14+CD16+ monocytes. Each data point is one individual
subject. (a). CCR2 on CD14+CD16+ monocytes by sex
(female: n = 24, male: n = 21). (b). CCR2 on CD14+CD16+
monocytes by ethnicity (Black: n = 18, Hispanic: n = 18, White: n = 9). (c).
CCR2 on CD14+CD16+ monocytes by detection of viral load (detectable:
n = 29, undetectable: n = 16). (d). Spearman’s rank correlation
between CCR2 on CD14+CD16+ monocytes and viral load
(log10) (n = 45, p = 0.92, ρ = −0.016). (e).
Spearman’s rank correlation between CCR2 on CD14+CD16+
monocytes and current CD4+ T cell counts (cells/μl) (n = 45, p = 0.18, ρ =
0.205). (f). Spearman’s rank correlation between CCR2 on
CD14+CD16+ monocytes and nadir CD4+ T cell counts
(cells/μl) (n = 45, p = 0.56, ρ = 0.088). (a–c). Data
are represented as median ± IQR. Significance was determined by Mann Whitney U
test. Data were not significant (p > 0.05).