Fig. 3. ¹H-MRS markers of neuronal health correlate inversely with CCR2 on CD14⁺CD16⁺ monocytes.

. Freshly isolated PBMC were stained for human CD14, CD16, and CCR2, or with isotype matched control antibodies. CCR2 MFI was calculated by subtracting the MFI of the IgG2b isotype matched control staining on CD14⁺CD16⁺ monocytes from the MFI of CCR2 on CD14⁺CD16⁺ monocytes. MRI/¹H-MRS imaging was performed within 1–2 weeks of blood draw. Pearson’s correlation coefficient was used to determine the coefficient of determination (r²). Each data point is one individual subject. (a). Inverse correlation of NAA/Cr in the right caudate nucleus with CCR2 on CD14⁺CD16⁺ monocytes (n = 18, p = 0.01, r² = 0.348). (b). Inverse correlation of Glx/Cr in the left caudate nucleus with CCR2 on CD14⁺CD16⁺ monocytes (n = 16, p = 0.01, r² = 0.356). (c). Inverse correlation of tCho/tCr in the right caudate nucleus with CCR2 on CD14⁺CD16⁺ monocytes (n = 18, p = 0.03, r² = 0.259).