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. Author manuscript; available in PMC: 2019 Jan 6.
Published in final edited form as: Leukemia. 2018 Jul 5;33(1):75–87. doi: 10.1038/s41375-018-0188-8

Figure 1. Generation and validation of BaF3 expressing WT and mutant G-CSFRs.

Figure 1

(a) Schematic of G-CSF/G-CSFR topology in the plasma membrane and sites of mutations found in the indicated disease phenotypes. (b) Flow cytometry plots of transduced BaF3 cells validating equivalent levels of G-CSFR expression after FACS-sorting (EV: empty vector, MIG: MSCVIRES-GFP). (c) Immunoblot analyses of the transduced BaF3 cells after 6 hour serum starvation and G-CSF stimulation (TAS: Time after Stimulation). The immunoblots shown here are representative of two independent experiments (n=2).