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. 2018 Dec 14;19(12):4046. doi: 10.3390/ijms19124046

Figure 1.

Figure 1

Schematic diagram of SlSBPASE gene structure, vector structure and CRSIPR/Cas9-induced mutagenesis. (A) Schematic diagram of SlSBPASE gene structure and target sequence. ATG, start codon; TGA, stop codon; sgRNA, single guide RNA; PAM, protospacer adjacent motif; AGG in red, PAM sequence. (B) Schematic diagram of the vector used in this study. LB, left border of T-DNA (Transfer DNA); RB, right border of T-DNA; U6, Arobidopsis U6 promoter; gRNA, guide RNA; e35S, enhanced 35S promoter; Cas9, optimized Cas9; NOS (nopaline synthase) Ter, NOS terminator; 35S, CaMV (Cauliflower mosaic virus) 35S promoter; HPT (hygromycin phosphotransferase), hygromycin selection marker; PolyA Ter, PolyA terminator. (C) Representative genotypes of slsbpase mutants. WT, wild type. Target sequences are in blue.