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Potential off-target sites and primers used in this study.
| Potential off-Target Sites | Primer Sequence (5′–3′) |
|---|---|
| Site 1 | F1: TGGGTCTGAATCATCACTAG |
| TGGGTCTGAATCATCACTAGAGG | R1: CTAGTGATGATTCAGACCCA |
| Site 2 | F2: AGTGCCTAAATCCTCAATAA |
| AGTGCCTAAATCCTCAATAAAGG | R2: TTATTGAGGATTTAGGCACT |
| Site 3 | F3: TGTGTCTAAATCATCATTCA |
| TGTGTCTAAATCATCATTCAAGG | R3: TGAATGATGATTTAGACACA |
