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. 2018 Dec 19;19(12):4118. doi: 10.3390/ijms19124118

Figure 5.

Figure 5

Transient expansion of T cell clones, present at high frequency within the tumor. (A): Deep sequencing of the TCRβ CDR3 regions as performed by Adaptive Biotechnologies (Seattle, WA, USA) on PBMCs collected one day prior to CAR T administration, then on post-infusion days 1, 8, 17, 24, 31, and 58. Deep sequencing data are presented as the productive frequency of clones containing the indicated amino acid sequences in the CDR3 region at each time point. The expansion of CAR T cells was determined by flow cytometry against antibody targeting the Vβ-20+ sequence in the CAR (dashed line). (B): Pair-wise scatter plot showing the productive frequency of the sum of frequencies of clones of T cells, based upon the CDR3 sequencing data. X-axis represents T cells collected from a scapular nodule on post-CAR day 10, while y-axis denotes peripheral blood collected on post-CAR day 8. The violet boxes denote preferential expansion of small numbers of T cell clones, denoted oligoclonal expansion.