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. 2018 Dec 11;19(12):3989. doi: 10.3390/ijms19123989

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Generation and molecular analysis of transgenic plants. (A) Schematic representation of the pBAR-GFP.UbiOPR3 expression cassette used for Sar-60 transformation. OsAct1, rice Actin 1 promoter; ZmUbi1, maize ubiquitin 1 promoter; NosT, nopaline synthase terminator; GFP, Green Fluorescent Protein gene; BAR, BASTA resistance gene (phosphinothricin acetyl transferase); AmpR, ampicillin resistance gene. (B) PCR analysis performed on the genomic DNA of T0 plants for the insertion of GFP gene (upper panel) and AtOPR3 (bottom panel). (C) End-point RT-PCR analysis on the total RNA of T0 plants (at heading stage) for the expression of the reference gene TaWIN1 (top panel), GFP gene (middle panel) and AtOPR3 (bottom panel). Lane M, DNA ladder as a molecular weight marker; Lane P, plasmid DNA pBAR-GFP.UbiOPR3; Lane C, non-transgenic wheat plant Sar-60; Lanes Tr-1–Tr-20 represent putative transgenic wheat plants.