Table 1.
Identification and analysis of proteins in leaves of cut lily after HRW, SNP, and HRW plus NaN3 or tungstate treatment.
| Spot No. | Protein Name | Species | Accession No. | Expressed MW (Da)/pI | Theoretical MW (Da)/pI | Peptide Count | Score | Protein Score Confidence level (C.I.%) |
Up/Down |
|---|---|---|---|---|---|---|---|---|---|
| 201 | ATP synthase CF1 alpha subunit (chloroplast) | Lilium superbum | YP_009130198.1 | 55,285.2/5.41 | 55,319.38/5.41 | 21 | 962 | 100 | ↑ |
| 259 | ATP synthase alpha subunit, partial (mitochondrion) | Erythronium dens-canis | AFM91753.1 | 28,195.8/6.51 | 28,213.43/6.50 | 10 | 114 | 100 | ↑ |
| 202 | ATP synthase CF1 alpha subunit (chloroplast) | Lilium superbum | YP_009130198.1 | 55,285.2/5.41 | 55,319.38/5.41 | 22 | 936 | 100 | ↑ |
| 908 | Photosystem II oxygen evolving complex protein 2 precursor | Fritillaria agrestis | AAC04809.1 | 28,094.2/8.31 | 28,111.52/8.31 | 5 | 60 | 98.435 | ↓ |
| 284 | ATP synthase CF1 beta subunit, partial (plastid) | Lilium superbum | AEZ48850.1 | 53,576.9/5.22 | 53,610.53/5.22 | 15 | 59 | 98.118 | ↑ |
| 294 | Ribulose-1,5-bisphosphate carboxulase/oxygenase large subunit, partial (chloroplast) | Heloniopsis kawanoi | AIW53238.1 | 50,960.6/6.23 | 50,992.85/6.24 | 29 | 1180 | 100 | ↑ |
| 180 | ATP synthase CF1 alpha subunit (chloroplast) | Lilium superbum | YP_009130198.1 | 55,285.2/5.41 | 5319.38/5.41 | 21 | 865 | 100 | ↑ |
| 896 | Carbonic anhydrase | Musa acuminata subsp. | Tr|M0TL28 | 22,422.1/5.07 | 22,436.69/5.06 | 4 | 104 | 99.985 | ↑ |
| 716 | PDZ domain-containing protein | Cynara cardunculus var. | Tr|A0A118JU51 | 36,198.3/6.18 | 36,220.78/6.18 | 6 | 113 | 99.998 | ↑ |
| 913 | Chlorophyll a-b binding protein, chloroplastic | Musa acuminata subsp. | Tr|M0SBM9 | 29,718.2/8.96 | 29,737.07/8.96 | 4 | 171 | 100 | ↑ |
| 431 | Actin | Lilium davidii var. Davidii | ALO18835.1 | 41,649.0/5.31 | 41,675.77/5.31 | 15 | 447 | 100 | ↑ |
| 511 | Glutamine synthetase | Tulipa pulchella | BAM84282.1 | 38,673.4/5.64 | 38,697.60/5.64 | 5 | 90 | 99.999 | ↑ |
| 479 | Actin | Lilium regale | AFU06383.1 | 41,619.0/5.31 | 41,645.75/5.31 | 16 | 421 | 100 | ↑ |
| 492 | Monodehydroascorbate reductase | Lilium longiflorum | ADF43731.1 | 46,732.5/5.89 | 46,761.56/5.89 | 14 | 86 | 99.996 | ↑ |
| 220 | ATP synthase CF1 alpha subunit (chloroplast) | Lilium superbum | YP_009130198.1 | 55,285.2/5.41 | 55,319.38/5.41 | 19 | 730 | 100 | ↓ |
| 988 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial (chloroplast) | Gagea wilczekii | AAM29162.1 | 50,739.4/5.96 | 50,771.69/5.96 | 12 | 494 | 100 | ↑ |
| 1060 | Pathogenesis-related protein 10 | Lilium regale | AHG94651.1 | 16,709.4/5.31 | 16,719.85/5.31 | 7 | 536 | 100 | ↓ |
| 136 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial (chloroplast) | Trillium camschatcense | AFP48691.1 | 44,763.6/6.52 | 44,792.01/6.53 | 9 | 63 | 99.14 | ↑ |
| 985 | ATP synthase beta subunit, partial (chloroplast) | Fritillaria acmopetala | AKG96681.1 | 51,914.1/5.13 | 51,946.57/5.13 | 16 | 68 | 99.774 | ↓ |
| 142 | ATP synthase CF1 alpha subunit (chloroplast) | Ripogonum album | ANO45506.1 | 55,341.1/5.26 | 55,375.27/5.26 | 10 | 72 | 99.91 | ↓ |
| 415 | Glutamine synthetase | Erythranthe guttata | Tr|A0A022RZ30 | 39,028.6/5.40 | 39,053.05/5.39 | 7 | 393 | 100 | ↓ |
| 405 | 6-Phosphogluconate dehydrogenase, decarboxylating | Citrus sinensis | Tr|A0A067G3F9 | 53,519.6/6.38 | 53,553.34/6.38 | 13 | 437 | 100 | ↓ |
| 444 | Elongation factor Tu | Vigna angularis var. Angularis | Tr|A0A0S3RGB1 | 52,659.2/6.34 | 52,692.26/6.34 | 14 | 671 | 100 | ↓ |
| 868 | Chlorophyll a-b binding protein, chloroplastic | Kalanchoe fedtschenkoi | Tr|A0A089WZX0 | 28,226.3/5.15 | 28,244.20/5.15 | 7 | 195 | 100 | ↑ |
| 517 | Glutamine synthetase | Lolium perenne | Tr|C5IW59 | 38,973.5/5.40 | 38,998.03/5.40 | 10 | 348 | 100 | ↓ |
| 866 | Chlorophyll a-b binding protein, chloroplastic | Carya cathayensis | Tr|Q1KLZ3 | 28,296.3/5.15 | 28,314.25/5.15 | 5 | 101 | 99.969 | ↑ |
| 880 | Beta carbonic anhydrase 3 | Arabidopsis thaliana | Sp|Q9ZUC2 | 28,810.8/6.54 | 28,829.03/6.54 | 6 | 94 | 99.83 | ↑ |
| 864 | Chlorophyll a-b binding protein, chloroplastic | Kalanchoe fedtschenkoi | Tr|A0A089WZX0 | 28,226.3/5.15 | 28,244.20/5.15 | 10 | 294 | 100 | ↓ |
| 860 | Carbonic anhydrase | Zea mays | Tr|Q41729 | 71,291.9/8.93 | 71,337.55/8.93 | 10 | 101 | 99.969 | ↓ |
| 542 | Ribulose bisphosphate carboxylase/oxygenase activase | Medicago truncatula | Tr|G7JTD2 | 52,135.9/5.42 | 52,169.06/5.42 | 15 | 358 | 100 | ↓ |
| 1037 | Type II peroxiredoxin | Medicago truncatula | Tr|A0A072U4Q3 | 25,893.6/9.35 | 25,909.84/9.35 | 10 | 191 | 100 | ↓ |
| 304 | ATP synthase CF1 beta subunit, partial (plastid) | Lilium superbum | AEZ48850.1 | 53,576.9/5.22 | 53,610.53/5.22 | 28 | 1190 | 100 | ↓ |
| 964 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial (chloroplast) | Cardiocrinum giganteum var. Yunnanense | AAM29161.1 | 50,201.1/6.04 | 50,233.05/6.04 | 12 | 88 | 99.998 | ↑ |
| 299 | ATP synthase CF1 beta subunit, partial (plastid) | Lilium superbum | AEZ48850.1 | 53,576.9/5.22 | 53,610.53/5.22 | 21 | 112 | 100 | ↓ |
| 130 | 70 kDa heat shock protein | Sandersonia aurantiaca | AAL85887.1 | 36,768.6/4.83 | 36,791.57/4.82 | 3 | 260 | 100 | ↓ |
| 633 | NADP-dependent alkenal double bond reductase P2 | Morus notabilis | Tr|W9SE47 | 40,693.7/6.23 | 40,719.76/6.22 | 7 | 96 | 99.89 | ↓ |
| 90 | Elongation factor G, mitochondrial | Medicago truncatula | Tr|A0A072UPP0 | 81,881.8/5.50 | 81,933.70/5.50 | 17 | 579 | 100 | ↓ |
| 671 | Cysteine synthase | Populus trichocarpa | Tr|B9HJY5 | 34,176.2/7.64 | 34,197.70/7.64 | 11 | 132 | 100 | ↑ |
| 839 | Putative L-ascorbate peroxidase 2, cytosolic-like | Solanum chacoense | Tr|A0A0V0HVQ3 | 28,638.6/5.75 | 28,656.76/5.75 | 9 | 324 | 100 | ↑ |
| 618 | Trypsin-like serine protease | Medicago truncatula | Tr|G7KIR6 | 45,774.3/6.79 | 45,802.44/6.80 | 10 | 525 | 100 | ↑ |
| 182 | FtsH-like protein Pftf | Nicotiana tabacum | Tr|Q9ZP50 | 74,335.8/6.00 | 74,382.14/6.00 | 24 | 658 | 100 | ↑ |
| 307 | ATP synthase alpha subunit, partial (mitochondrion) | Lilium lancifolium | AAR28047.1 | 41,824.8/6.47 | 41,850.81/6.47 | 13 | 397 | 100 | ↑ |
| 313 | Atpb (chloroplast) | Lilium distichum | AMT85217.1 | 53,546.9/5.22 | 53,580.50/5.22 | 17 | 113 | 100 | ↓ |
| 580 | Glyceraldehyde-3-phosphate dehydrogenase, partial | Lilium regale | AHZ94971.1 | 36,779.2/7.11 | 36,802.04/7.11 | 12 | 255 | 100 | ↑ |
| 349 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial (chloroplast) | Cardiocrinum giganteum var. Yunnanense | AAM29161.1 | 50,201.1/6.04 | 50,233.05/6.04 | 19 | 159 | 100 | ↑ |
| 489 | Monodehydroascorbate reductase | Lilium longiflorum | ADF43731.1 | 46,732.5/5.89 | 46,761.56/5.89 | 18 | 271 | 100 | ↓ |
| 752 | Photosystem II oxygen evolving complex protein 1 precursor | Fritillaria agrestis | AAC04808.1 | 34,847.8/6.26 | 34,869.39/6.25 | 16 | 695 | 100 | ↓ |
| 263 | Dihydrolipoyl dehydrogenase | Salvia miltiorrhiza | Tr|A0A0G2SJN7 | 53,520/6.96 | 53,553.72/6.96 | 6 | 259 | 100 | ↑ |
| 192 | Malic enzyme | Phaseolus angularis | Tr|A0A0L9UG31 | 73,189.2/8.33 | 73,235.49/8.33 | 9 | 177 | 100 | ↓ |
| 604 | Fructose-bisphosphate aldolase | Oxytropis ochrocephala | Tr|A0A0K1JSG5 | 42,894.2/6.39 | 42,920.76/6.39 | 9 | 477 | 100 | ↑ |
Note: Assigned spot number as indicated in Figure 3. Arrows indicate up- (↑) and down- (↓) regulation of the proteins.