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. 2018 Nov 30;23(12):3144. doi: 10.3390/molecules23123144

Figure 3.

Figure 3

MALT1 is not necessary for RANK-induced NF-ĸB and AP-1 signaling. MALT1-deficient HEK293T cells or cells reconstituted with MALT1 (as indicated on the X-axis) were transiently transfected with a RANK expression plasmid, a plasmid constitutively expressing β-galactosidase, and plasmids expressing either an NF-κB-dependent or AP-1-dependent luciferase reporter gene. The / represents set-ups where neither RANK nor MALT1 is expressed. Statistical differences were determined by Student’s t-test, *** represents p ≤ 0.001. (a) Expression of MALT1 and RANK was verified by Western blotting. The four left lanes correspond to the samples analyzed in (b) for NF-κB reporter activation, the last four lanes correspond to the samples analyzed in (b) for AP-1 reporter activation. (b) Luciferase activity was measured 24 h after transfection and normalized to β-galactosidase expression (plotted as luc/gal). Values are the mean of triplicates ± S.D. Data shown are representative of two independent experiments.