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Solubilization of microvillus membrane vesicles with NaC or DDM at concentrations ranging from 0–10 mM as described in Methods. After centrifugation, the pellet (P) and supernatant (S) fractions were subjected to SDS/PAGE and proteins visualized by staining with Coomassie brilliant blue. Arrows indicate the molecular mass-values of the brush border proteins sucrase-isomaltase (250 kDa), ApN (150 kDa), actin (42 kDa), and annexin A2 (36 kDa).
