Table 1.
Assay | Main Mechanism | Oxidant | Probe | Detection | Ref. |
---|---|---|---|---|---|
ORAC | HAT | ROO∙ | FL | Fluorescence | [1,7] |
DPPH | SET | DPPH∙ | DPPH∙ | Absorbance | [8,9] |
FRAP | SET | Fe3+ | [Fe(TPTZ)2]2+ | Absorbance | [10] |
TEAC | SET | ABTS∙+ | ABTS+ | Absorbance | [11,12] |
HORAC | HAT | HO∙ | FL | Fluorescence | [13] |
TRAP | HAT | ROO∙ | β-PE | Fluorescence | [14,15] |
CUPRAC | SET | Cu2+ | Neocuproine | Absorbance | [16] |
Total Phenolic Assay | SET | FCR | FCR | Absorbance | [17] |
Crocin Bleaching | HAT | ROO∙ | Crocin | Absorbance | [18] |
Chemiluminescence | HAT | H2O2 | Luminol | Fluorescence | [19] |
ABTS∙+: 2,2′-azino-bis (3-ethylbenzothiazoline-6- sulfonic acid) radical cation; CUPRAC: Cupric Ion Reducing Antioxidant Capacity; DPPH: 2,2-Diphenyl-1-picrylhydrazyl; DPPH∙: DPPH radical; FCR: Folin Ciocalteu Reagent; [Fe(TPTZ)2]2+: 2,4,6-Tris(2-pyridyl)-s-triazine complex; β-PE: β-phycoerythrin; FL: Fluorescein; FRAP: Ferric Ion Reducing Antioxidant Power; HORAC: Hydroxyl (HO) Radicals Averting Capacity; ORAC: Oxygen Radical Absorbance Capacity; ROO∙: peroxylradical generated by 2,2′-Azobis(2-methylpropionamidine) dihydrochloride; TEAC: Trolox Equivalent Antioxidant Capacity; TRAP: Total Radical-trapping Antioxidant Parameter.