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. 2018 Dec 7;23(12):3244. doi: 10.3390/molecules23123244

Table 1.

List of the widespread assays to evaluate Antioxidant Capacity.

Assay Main Mechanism Oxidant Probe Detection Ref.
ORAC HAT ROO∙ FL Fluorescence [1,7]
DPPH SET DPPH∙ DPPH∙ Absorbance [8,9]
FRAP SET Fe3+ [Fe(TPTZ)2]2+ Absorbance [10]
TEAC SET ABTS∙+ ABTS+ Absorbance [11,12]
HORAC HAT HO∙ FL Fluorescence [13]
TRAP HAT ROO∙ β-PE Fluorescence [14,15]
CUPRAC SET Cu2+ Neocuproine Absorbance [16]
Total Phenolic Assay SET FCR FCR Absorbance [17]
Crocin Bleaching HAT ROO∙ Crocin Absorbance [18]
Chemiluminescence HAT H2O2 Luminol Fluorescence [19]

ABTS∙+: 2,2′-azino-bis (3-ethylbenzothiazoline-6- sulfonic acid) radical cation; CUPRAC: Cupric Ion Reducing Antioxidant Capacity; DPPH: 2,2-Diphenyl-1-picrylhydrazyl; DPPH∙: DPPH radical; FCR: Folin Ciocalteu Reagent; [Fe(TPTZ)2]2+: 2,4,6-Tris(2-pyridyl)-s-triazine complex; β-PE: β-phycoerythrin; FL: Fluorescein; FRAP: Ferric Ion Reducing Antioxidant Power; HORAC: Hydroxyl (HO) Radicals Averting Capacity; ORAC: Oxygen Radical Absorbance Capacity; ROO∙: peroxylradical generated by 2,2′-Azobis(2-methylpropionamidine) dihydrochloride; TEAC: Trolox Equivalent Antioxidant Capacity; TRAP: Total Radical-trapping Antioxidant Parameter.