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Co-localization of GmWRKY12. The recombinant plasmid of GmWRKY12-GFP and At2G03340-mCherry were co-transformed into wheat mesophyll protoplasts under the control of the CaMV 35S promoter. GmWRKY12 was localized in the nucleus of wheat mesophyll protoplasts protoplasts. Results were observed by a confocal laser scanning microscope (LSM700; CarlZeiss, Oberkochen Germany) after incubating in darkness at 22 °C for 18–20 h. Scale bars = 10 μm.
