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. 2018 Nov 26;19(12):3755. doi: 10.3390/ijms19123755

Figure 2.

Figure 2

Use of the Thermal Shift Assay and Isothermal Titration Calorimetry to identify ligands that bind to AdmX. (A) Domain arrangement of AdmX and its ligand binding domain (LBD). (B–E) Thermal shift experiments of AdmX and AdmX-LBD in the absence and presence of indole-3-acetic acid (IAA). (B,C) raw data; (D,E) first derivatives of raw data; (F,G) microcalorimetric titrations of buffer, AdmX and AdmX-LBD with IAA. Upper panel: titration raw data; lower panel: fit of dilution heat-corrected and concentration-normalized raw data with a model for the binding of a single ligand to a macromolecule. The derived thermodynamic binding parameters are indicated.