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. 2018 Dec 14;19(12):4059. doi: 10.3390/ijms19124059

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Phylogenetic tree (left) and predicted conserved motifs (right) of ScWRKY3 protein and WRKYs from various plant species. The GenBank accession number of WRKY proteins follows the protein name. Ml, Miscanthus lutarioriparius; Sb, Sorghum bicolor; Zm, Zea mays; Si, Setaria italic; Os, Oryza sativa; and At, Arabidopsis thaliana. The unrooted tree is constructed by the Maximum Likelihood with bootstrapping (1000 iterations) using MEGA7.0 software. ScWRKY3 is underlined. The conserved domains were predicted by MEME Suite 5.0.2 software. The different-colored boxes named at the bottom represent conserved motifs. Gray lines represent the nonconserved sequences, and the position of each WRKY sequence is exhibited proportionally. The motif logo is shown in Figure S6.