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. 2018 Dec 14;19(12):4059. doi: 10.3390/ijms19124059

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of transient overexpression of ScWRKY3 in Nicotiana benthamiana leaves. (A) Semi-quantitative PCR analysis of ScWRKY3 in N. benthamiana leaves after one day of infiltration by Agrobacterium strain GV3101 carrying pEarleyGate 203-ScWRKY3 (35S::ScWRKY3) and the empty vector pEarleyGate 203 (35S::00). (B) Phenotype of N. benthamiana leaves after one day of agroinfiltration. (C) The transcript level of nine immunity-associated marker genes in the N. benthamiana leaves after one day of agroinfiltration. (D,F) Disease symptoms of N. benthamiana post-inoculation with Ralstonia solanacearum and Fusarium solani var. coeruleum are observed after one day and seven days of agroinfiltration. (E,G) The transcripts of nine immunity-associated marker genes in the N. benthamiana leaves after inoculation with R. solanacearum or F. solani var. coeruleum for one day and seven days. Data are normalized to the NtEF-1α expression level. All data points are means ± standard error (n = 3). Bars superscripted by different lowercase letters indicate significant differences, as determined by Duncan’s new multiple range test (p value < 0.05).

Effects of transient overexpression of ScWRKY3 in Nicotiana benthamiana leaves. (A) Semi-quantitative PCR analysis of ScWRKY3 in N. benthamiana leaves after one day of infiltration by Agrobacterium strain GV3101 carrying pEarleyGate 203-ScWRKY3 (35S::ScWRKY3) and the empty vector pEarleyGate 203 (35S::00). (B) Phenotype of N. benthamiana leaves after one day of agroinfiltration. (C) The transcript level of nine immunity-associated marker genes in the N. benthamiana leaves after one day of agroinfiltration. (D,F) Disease symptoms of N. benthamiana post-inoculation with Ralstonia solanacearum and Fusarium solani var. coeruleum are observed after one day and seven days of agroinfiltration. (E,G) The transcripts of nine immunity-associated marker genes in the N. benthamiana leaves after inoculation with R. solanacearum or F. solani var. coeruleum for one day and seven days. Data are normalized to the NtEF-1α expression level. All data points are means ± standard error (n = 3). Bars superscripted by different lowercase letters indicate significant differences, as determined by Duncan’s new multiple range test (p value < 0.05).