Figure 6.

The bL compound inhibits mammosphere formation. (A) MDA-MB-231 and two independent clones of NQO1 stable cells (NQO1 #1 and #2) were induced to mammosphere formation for one and two weeks, as described in Section 4. After mammosphere formation, bL (2 µM) was added for 24 h. Images were taken using a new hybrid microscope (Echo, San Diego, CA, USA). The scale bar is 200 μm. (B) Cell lysates obtained from the mammosphere formation assay were subjected to Western blot analysis using the indicated antibodies; β-tubulin was used as a loading control.