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. 2018 Dec 17;19(12):4078. doi: 10.3390/ijms19124078

Figure 5.

Figure 5

Pharmaceutical Doxycycline (DOX) Directly Scavenges Superoxide (O2•−) and Hydrogen Peroxide (H2O2). (A) EPR spectroscopy was performed in a O2•−generating reaction containing 50 μM hypoxanthine (HX), 10 mU/mL of xanthine oxidase (XO), and 200 µM of CMH at 37 °C for 30 min. To determine the ability of DOX to scavenge O2•− the reactions were performed in the presence of absence 1 mg/mL pharmaceutical DOX. Control reactions included SOD, a known direct scavenger of O2•−. (B) The ability of pharmaceutical DOX to scavenge H2O2 was determined by EPR spectroscopy in KDD(+) buffer containing 10 μM H2O2 in the presence or absence of 1 mg/mL pharmaceutical DOX at 37 °C for 30 min. Control reactions included catalase (CAT), a known direct scavenger of H2O2. (* p < 0.05) vs. CMH (A) or KDD(+) and CMH (B), (≠ p < 0.05) vs. CMH, HX, and XO (A) or KDD(+), CMH, and H2O2 (B). N = 3–6.