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. 2018 Dec 9;19(12):3957. doi: 10.3390/ijms19123957

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The miR-23a~27a~24-2 cluster represses preadipocyte differentiation. (A–C) Time course of miR-23a, miR-27a, and miR-24-2 expression during preadipocyte differentiation. (D) Oil red O staining of lipids in differentiated adipocytes transfected with miR-23a agomir (23m)/antagomir (23I), miR-27a agomir (27m)/antagomir (27I), miR-24-2 agomir (24-2m)/antagomir (24-2I), negative control (NC) for agomir (mNC) and NC for antagomir (INC, magnification: 40×). (E) ELISA analysis of TG content in adipocytes transfected with Agomir NC, miR-23a agomir, miR-27a agomir and miR-24-2 agomir. Error bars represent SEM. Lowercase represents p < 0.05. Uppercase represents p < 0.01. “*” represents p < 0.05; “**” represents p < 0.01.