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. 2018 Dec 9;19(12):3957. doi: 10.3390/ijms19123957

Figure 3.

Figure 3

The miR-23a~27a~24-2 cluster negatively regulates the expression of ATP5F1, DCN, GPAM, DGAT2, G6PD, and LPL. (A–C) Time course of ATP5F1, DCN, GPAM, DGAT2, G6PD, and LPL expression during preadipocyte differentiation. (D–F) mRNA expression of ATP5F1, DCN, GPAM, DGAT2, G6PD, and LPL in preadipocytes individually transfected with miR-23a agomir, miR-27a agomir, and miR-24-2 agomir, followed by adipogenic differentiation at 36 h. (G–I) Transfection of miR-23a antagomir, miR-27a antagomir, and miR-24-2 antagomir in preadipocytes, followed by adipogenic differentiation increased ATP5F1, DCN, GPAM, DGAT2, G6PD, and LPL expression from 36 h to 8 days. Error bars represent SEM. Lowercase represents p < 0.05. Uppercase represents p < 0.01. “**” represents p < 0.01.