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. 2018 Dec 9;19(12):3957. doi: 10.3390/ijms19123957

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Interference effects of DCN, GPAM, DGAT2, G6PD, and LPL on adipocyte adipogenesis. (A) Bright field microscopy of lipid droplets in DCN- and LPL-interfered preadipocytes followed by differentiation at day 5 (magnification: 100×). As there were no obvious lipid droplets observed in the bright field microscopy when interfering GPAM, DGAT2, and G6PD, the data was not shown. (B–C) Oil Red O staining of lipids (magnification: 40×) and ELISA analysis of TG content showed that DCN, G6PD, and LPL were strongly anti-adipogenic while GPAM and DGAT2 were pro-adipogenic in adipogenesis. Error bars represent SEM. “*” represents p < 0.05.