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. 2018 Dec 11;19(12):3994. doi: 10.3390/ijms19123994

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Depigmenting effect of compound 8 and melanogenic regulators on melanogenesis of zebrafish in an in vivo phenotype-based system. Zebrafish embryos were exposed to (A) E3 buffer (containing 1% alcohol), (B) arbutin (20 μM), or (C) compound 8 (20 μM) from 7 hpf (post-fertilization) to 72 hpf. (D) Pigmentation levels of zebrafish treated with arbutin (20 mM and 20 μM) and compound 8 (10 μM and 20 μM). Results were expressed as % control and data mean ± S.D. n = 15 in each group. * p < 0.05 and *** p < 0.001 compared to the control group.