qRT-PCR validation of illumina Hiseq findings and screening of damage severity prediction marker genes. Values represent means ± SE (n = 3) and the significance level of 0.05 was used for different letters above bars. Genes have continual increasing or decreasing expression patterns were selected as candidate genes. a: VIT_05s0020g03740.t01, b: VIT_16s0050g02530.t01 c: VIT_19s0015g01070.t01, d: VIT_05s0049g00520.t01, e: VIT_13s0067g02360.t01, f: VIT_12s0035g01910.t01, g: VIT_04s0023g00530.t01, h: VIT_05s0049g01070.t01, i: VIT_06s0004g05670.t01, j: VIT_10s0003g01810.t01, k: VIT_00s0332g00110.t01, l: VIT_00s0201g00080.t01, m: VIT_07s0005g00160.t01, n: VIT_11s0052g01180.t01, o: VIT_14s0128g00020.t01, p: VIT_05s0062g00300.t01.