. 2018 Dec 13;19(12):4029. doi: 10.3390/ijms19124029

Table 2.

Melatonin supplementation on parthenogenetic development of cryopreserved mouse MII oocytes.

Groups	No. of Oocytes Activated	No. of Activated Oocytes Developed to
Groups	No. of Oocytes Activated	2-Cell Embryos (%)	4-Cell Embryos (%)	Morula (%)	Blastocysts (%)	Hatched Blastocysts (%)
Control	150	141 (94.00 ± 2.55) ^a	140 (93.33 ± 0.87) ^a	129 (86.00 ± 1.94) ^a	100 (66.67 ± 1.32) ^a	50 (33.33 ± 15.35) ^a
Vitrfication	122	90 (73.77 ± 11.96) ^b	97 (79.51 ± 11.96) ^b	80 (65.57 ± 12.32) ^b	41 (33.61 ± 6.54) ^b	8 (6.56 ± 4.78) ^c
Vitrification + MT	175	160 (91.43 ± 9.62) ^a	165 (94.29 ± 10.06) ^a	147 (84.00 ± 7.72) ^a	100 (57.14 ± 16.17) ^a	39 (22.29 ± 3.82) ^b

The rates of 2-cell, 4-cell, morula, blastocysts and hatched blastocysts were calculated from the total number of activated oocytes. For instance, the rate of 2-cell embryos (%) = (No. of zygotes cleaved/No. of oocytes activated) × 100. The values indicate the mean ± SD of five independent experiments. Values with different superscripts (a, b and c) in the same column are significantly different (p < 0.05).