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PCR primers used for SYBR green Q-PCR analysis.
| Gene | Assay ID | Primer seq (5′-3′) | Product Length | Tm (°C) |
|---|---|---|---|---|
| P53 | NM_001127233.1 | F: AGGATTGTGGCCTTCTTTGA | 126 | 62 |
| R: CAGATGCCGGTTCAGGTACT | ||||
| P21 | NM_001111099.2 | F: TGGAGATGAACTGGACAGCA | 84 | 62 |
| R: TGAAGTTGCCATCAGCAAAC | ||||
| E2F1 | NM_001291105.1 | F: CGAGTCCTATGCCTTCAACA | 159 | 62 |
| R: GAGTCCAGCCAGGAGATGAC | ||||
| GAPDH | NM_001289726.1 | F: AGAACATCATCCCTGCATCC | 124 | 62 |
| R: AGATCCACGACGGACACATT |
