Table 1.
Primers used in this study.
| Gene | Primer | Sequence (5′–3′) | Product Size (bp) |
|---|---|---|---|
| TNF-α | Forward | TGGGCTCCAAGCATCCAACT | 182 |
| Reverse | GGCCCTCACATTCTGGGTGT | ||
| IL-1β | Forward | TCGAAACGTCCTCCGACGAG | 131 |
| Reverse | TGAGAGGAGGTGGAGAGCCT | ||
| IL-6 | Forward | CAAGCGCCTTCACTCCATTC | 176 |
| Reverse | GATTTTGTCGACCATGCGCT | ||
| TAP | Forward | CGCTCCTCTTCCTGGTCCTG | 197 |
| Reverse | TGATCCCGGCTGTGTCTTGG | ||
| Bac5 | Forward | CAGTCACCCTGGACCCATCA | 124 |
| Reverse | GGGCGGAACGGTGGATAGAA | ||
| 18S rRNA | Forward | AGTGGAGCCTGCGGCTTAAT | 105 |
| Reverse | CACCACCCACGGAATCGAGA |
TNF-α—tumor necrosis factor alpha; IL—interleukin; TAP—tracheal antimicrobial peptide; Bac5—bactenecin 5; rRNA—ribosomal RNA.