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. 2018 Dec 3;19(12):3853. doi: 10.3390/ijms19123853

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Comparison of behavior endpoints between wild-type and C₆₀ NPs-exposed zebrafish in novel tank exploration after five-day exposure. (A) Average speed, (B) freezing time movement ratio, (C) time in top duration, (D) number of entries to the top, (E) latency to enter the top, and (F) total distance traveled in the top were analyzed. The black line represented control and red line represented C₆₀ NPs-exposed fish in Figure 3A-F. The swimming trajectories of control (black color in (G,H)) and C₆₀ NPs-exposed fish (red color in (I,J)) for novel tank exploration test after 1 min and 15 min were recorded and compared. The data are expressed as the mean ± SEM and were analyzed by Mann–Whitney test (n = 30 for control; n = 30 for C₆₀ NPs-exposed fish; * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.0001).