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. 2018 Dec 3;19(12):3853. doi: 10.3390/ijms19123853

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Comparison of mirror biting behavior endpoints between wild-type and C₆₀ NPs-exposed zebrafish after seven-day exposure. (A) Average speed, (B) mirror biting time percentage, (C) longest duration in the mirror side, (D) freezing time movement ratio, (E) swimming time movement ratio, and (F) rapid movement time ratio were analyzed. The swimming trajectories of control (black color in (G)) and C₆₀ NPs-exposed fish (red color in (H)) for mirror biting test were recorded and compared. The data are expressed as the means and were analyzed by Mann–Whitney test (n = 30 for control; n = 30 for C₆₀ NPs-exposed fish; *** p < 0.001, **** p < 0.0001).