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. 2018 Dec 3;19(12):3853. doi: 10.3390/ijms19123853

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Comparison of conspecific behavior endpoints between wild-type and C₆₀ NPs-exposed zebrafish after nine-day exposure. (A) Interaction time percentage, (B) longest duration in separator side, (C) average speed, and (D) average distance to separator were analyzed. The swimming trajectories of control (black color in (E)) and C₆₀ NPs-exposed fish (red color in (F)) for social interaction test were recorded and compared. The data are expressed as the means and were analyzed by Mann–Whitney test (n = 60 for control; n = 24 for C₆₀ NPs-exposured fish; ** p < 0.01).