Figure 9.

The circadian rhythm assay for wild-type and C₆₀ NPs-exposed zebrafish after 12-day exposure. Comparison of the time chronological changes of the average speed (A) and meandering (B) between wild-type and C₆₀ NPs-exposed fish in day and night cycle. The grey area shows the dark period and the unshaded area is the light period. Comparison of the average speed (C), average angular velocity (D), and meandering (E) at day cycle. Comparison of the average speed (F), average angular velocity (G), and meandering (H) at night cycle. The data are expressed as the mean ± SEM and were analyzed by Mann–Whitney test (n = 28 for control; n = 18 for C₆₀ NPs-exposed fish; *** p < 0.005, ****, p < 0.0001).