Table 2.
Detection of biochemical parameters in the brain tissue for C60 NPs-exposed zebrafish after 12-day exposure.
| Biomarker | Control | C60 (1ppm) | C60 (2ppm) | Unit | Significance | ANOVA F value | p value |
|---|---|---|---|---|---|---|---|
| ACh | 22.750 ± 0.373 a | 15.520 ± 1.686 b | 9.309 ± 0.641 c | U/μg totalprotein | YES | F (2, 6) = 39.99 | p = 0.0003 |
| AChE | 3.102 ± 0.079 a | 3.272 ± 0.504 a | 7.527 ± 0.284 b | U/μg totalprotein | YES | F (2, 6) = 55.34 | p = 0.0001 |
| Dopamine | 11.860 ± 2.769 a | 11.940 ± 1.420 a | 34.530 ± 5.335 b | pg/μg totalprotein | YES | F (2, 6) = 13.42 | p = 0.0061 |
| GABA | 0.057 ± 0.003 a | 0.057 ± 0.001 a | 0.159 ± 0.013 b | ug/μg totalprotein | YES | F (2, 6) = 59.43 | p = 0.0001 |
| melatonin | 3.459 ± 0.143 a | 1.759 ± 0.035 b | 2.493 ± 0.301 b | pg/μg totalprotein | YES | F (2, 6) = 19.43 | p = 0.0024 |
| glutamate | 0.633 ± 0.069 a | 0.392 ± 0.024 a,b | 0.379 ± 0.055 b | ug/μg totalprotein | YES | F (2, 6) = 7.312 | p = 0.0246 |
Enzymatic or ELISA-based methods were applied to detect the ACh (acetylcholine), AChE (acetylcholinesterase), dopamine, GABA (gamma-aminobutyric acid), melatonin, and glutamate. Data were presented as means ± S.E.M with three to six independent experiments. Statistical analysis was by one-way ANOVA test followed by the Tukey post-hoc test. Different labels above columns indicate a significant difference between different experimental groups with p < 0.01.