Figure 1.

Mouse splenic B cells cultured ex vivo with lipopolysaccharide (LPS) differentiate into antibody-secreting cells (ASCs) and experience changes in splicing and gene expression. The expression of the ELL family members changes from primarily ELL1 and ELL3 in B cells to induction of large amounts of ELL2 in ASCs (not shown). The exon splicing patterns are figuratively meant to show the increasing complexity seen in cells with time. We hypothesize that the decrease of RNA for SnapC and snRNA is linked to the regulated Ire1-dependent mRNA decay pathway (RIDD) activity induced by endoplasmic reticulum (ER) stress. About 4211 genes spliced early (18 h) persist into the 72 h samples and are seen as ELL2 independent. Meanwhile 7361 genes alternatively spliced at 18 h are missing at 72 h and are replaced by the 5115 genes that are ELL2 dependent.