Figure 8.
TXA1.HCl treatment induced autophagy. NCI-H460 cells were treated with 6.9 µM or with 9.7 µM TXA1.HCl for 48 h or with the solvent control (H2O). (a) PARP-1 and LC3 levels were analyzed by Western blot. Actin was used as loading control. (b) Programmed cell death was assessed with the TUNEL assay. (c) Levels of p70 s6K protein and of the phosphorylated form were analyzed by Western blot. Actin was used as a loading control. (d) Cellular proliferation was evaluated with the BrdU incorporation assay followed by fluorescence microscopy. In blue, Dapi stained nuclei. In green, BrdU incorporating nuclei. Values in the images correspond to the % of proliferating cells, expressed as mean ± SEM (* p < 0.05 vs. control). Results (and representative images) are from at least 3 independent experiments, except when otherwise stated (which were from 2 experiments only).