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. 2018 Dec 5;19(12):3886. doi: 10.3390/ijms19123886

Figure 1.

Figure 1

The effect of A769662 on insulin-stimulated NO production and Akt phosphorylation in HUVECs. (A) HUVECs were stimulated with A769662 (50 µM, 45 min) prior to insulin (1 µM, 15 min), conditioned media collected and NO production assessed. Data (mean ± SEM) shown from three independent replicates. (B–E) HUVECs were stimulated with A769662 (50 µM, 45 min) or AICAR (2 mM, 45 min) prior to insulin (51 µM, 15 min) and cell lysates prepared. Proteins were resolved by SDS-PAGE and immunoblotted with the antibodies indicated. (B) Representative immunoblots from three biological replicates with molecular weight markers indicated. Phospho-Akt Thr308 protein levels were assessed by stripping and re-probing the membranes. Densitometric quantification of (C) ACC, (D) Akt Ser473 and (E) Akt Thr308 phosphorylation normalised to β-tubulin or Akt (mean ± SEM). * p < 0.05, ** p < 0.01, relative to absence of insulin. # p < 0.05, ## p <0.01 relative to absence of AMPK activator.