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. 2018 Dec 5;19(12):3886. doi: 10.3390/ijms19123886

Figure 5.

Figure 5

The effect of A769662 on insulin-stimulated NO synthesis and signalling in HAECs. (A) HAECs were stimulated with the indicated concentrations of A769662 prior to insulin stimulation (1 µM, 15 min). Conditioned media was collected and NO production assessed. (B–D) HAECs were incubated with 50 µM A769662 for 45 min prior to insulin stimulation (1 µM) for the indicated durations. Cell lysates were prepared and immunoblotted with the antibodies indicated. (B) Representative immunoblots from three independent biological replicates with molecular weight markers indicated. (C,D) Densitometric quantification (mean ± SEM) of (C) Akt Ser473 or (D) Erk1 Thr202/Tyr204 phosphorylation normalised to total Akt or Erk1 levels respectively (mean ± SEM). * p < 0.05, ** p < 0.01 relative to absence of insulin. # p < 0.05, relative to absence of A769662.