Figure A4.

Averaged super-resolved DNA-PAINT images of DNA origami nanostructures for the whole time series of 83 min. The total numbers of identified DNA origami nanostructures $n_{\mathrm{ns}}$ employed for averaging are stated above the average images. Line profiles of total number of localizations through the two lower left points from each averaged image were fitted with a double Gaussian function $\left(f\left(x\right)=a_1\exp \left[\frac{{\left(x-b_1\right)}^2}{2{\sigma }_1^2}\right]+a_2\exp \left[\frac{{\left(x-b_2\right)}^2}{2{\sigma }_2^2}\right]+c\right)$. The number stated for each peak is the FWHM (in nm) and can be interpreted as a measure of the achievable resolution; the peak-to-peak distance is shown above the plot. Additionally, the value of the nearest neighbor analysis (NeNA) [11,75] is shown for the first subset.