Table 1.

Binding kinetics as measured by SI-FCS titrations of the imager concentrations $\langle A\rangle$. Hybridization rates and affinity as obtained from the titrations in Figure 4. The errors represent the 95% confidence bounds of the fit.

Imaging Condition	${\mathit{k}}_{\mathbf{a}}[{10}^6·{\mathbf{Ms}}^{-1}]$	${\mathit{k}}_{\mathbf{d}}[{\mathbf{s}}^{-1}]$	${\mathit{K}}_{\mathbf{d}}[\mathbf{nM}]$
conventional	$1.49\pm 0.17$	$0.303\pm 0.010$	$200\pm 30$
PO+C	$1.5\pm 0.4$	$0.268\pm 0.017$	$180\pm 60$
PCD+PCA	$1.3\pm 0.4$	$0.25\pm 0.02$	$190\pm 70$
18-mer spacer	$0.59\pm 0.11$	$0.245\pm 0.012$	$420\pm 100$
18-mer spacer with PO+C	$0.28\pm 0.12$	$0.26\pm 0.02$	$1000\pm 500$