Experimental design and effect of 25, 50, and 100 mg/kg doses of I. asarifolia (IA) aqueous extract and 250 mg/kg of sulfasalazine (SSZ) on disease activity index (DAI) (a); macroscopic damage index (MDI) (b); experimental design (c); weight/length relationship of the colon (d) of the different groups in the experimental trial of acute colitis induced by 2,4-dinitrobenzene sulphonic acid (DNBS) in rats. H, Healthy group; DNBS, DNBS group; SSZ, Sulfasalazine group; and the 25, 50, and 100 mg/kg doses of IA. Data are expressed as mean ± SEM, (n = 8). Statistical differences * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 of treatments vs. DNBS control were presented.