Skip to main content
. 2018 Dec 11;19(12):3985. doi: 10.3390/ijms19123985

Figure 1.

Figure 1

Transcription of Sm-MIR408 is induced under deficient copper conditions or NaCl treatment. (A) Expression level of Sm-MIR408 when two-week-old S. miltiorrhiza seedlings were transferred to standard Murashige and Skoog (MS) medium (0.1 μM copper), MS medium deficient in copper (0 μM, MS−Cu) and sufficient copper (5 μM, MS+Cu) for 7 days. (B) β-glucuronidase (GUS) staining results when one-week-old transgenic Nicotiana benthamiana expressing MIR408pro::GUS were transferred to MS, MS−Cu and MS+Cu for three weeks. (C) Expression levels of Sm-MIR408 in one-month-old Salvia miltiorrhiza seedlings treated with 150 mM NaCl for 24 h. (D) The GUS staining in the transgenic Nicotiana benthamiana expressing MIR408pro::GUS after treatment with 150 mM NaCl. For RT-qPCR, all data are means of three biological replicates, with error bars indicating SD; significant differences were determined using Duncan’s multiple range test (indicated by different letters at p < 0.05).