Figure 4.

Contents of reactive oxygen species (ROS) in wild type (WT) and miR408-overexpressing Nicotiana benthamiana. (A) 3,3-Diaminobenzidine (DAB) and nitroblue tetrazolium (NBT) staining when three-week-old seedlings were treated with 150 mM NaCl for 10 days. The strength of color showed the concentration of H₂O₂ and O₂⁻. (B) Contents of H₂O₂ in tobacco with or without salt treatment. (C–E) The activities of SOD (C), POD (D), and CAT (E) in WT and transgenic lines when three-week-old seedlings were treated with 150 mM NaCl for 10 days. (F–H) Expression of ROS-responsive genes NbSOD (F), NbPOD (G), and NbCAT (H), determined via RT-qPCR. The data represent means ± SD of three independent experiments. Significant differences were determined using Duncan’s multiple range test (indicated by different letters at p < 0.05).