Figure 5.

MTZ concentrations in the skin as a whole, the viable epidermis and dermis (VED), and the stratum corneum after the topical application of Pal-GH gel formulations on hairless rat skin. Significant differences were observed between F5_MTZ, F5_PG-MTZ, F5_IPM-MTZ, and 1% solution of MTZ in the whole skin, and stratum corneum (* p < 0.05). Additionally, significant differences were observed between F5_PG-MTZ and F5_IPM-MTZ with a 1% solution of MTZ and between F5_IPM-MTZ with F5_MTZ in the VED (* p < 0.05). The MTZ concentration of the stratum corneum was estimated by dividing the MTZ difference (between the VED and whole skin) by the weight of the stratum corneum. Closed columns: 1% MTZ solution, open columns: F5_MTZ, horizontal striped columns: F5_PG-MTZ, striped columns: F5_IPM-MTZ.